IBI GEL/PCR FRAGMENT EXTRACTION KIT-100 PREPS

Efficient Gel Extraction and PCR Clean-Up

The Gel/PCR DNA Fragment Extraction Kits are designed to recover or concentrate DNA
fragments (50bp-10kb) from an agarose gel, PCR, or any other enzymatic reaction. This method
uses a chaotropic salt to dissolve the agarose gel and denature the enzymes. The DNA fragments
in the chaotropic salt are bound to the glass-fiber matrix of the spin column. The contaminants
are washed with a wash buffer (containing Ethanol) and the purified DNA fragments are eluted
by a low salt based elution buffer or water. Salts, enzymes and unincorporated nucleotides can
be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.
Typically, recoveries are 80-90% for Gel Extraction and 90-95% for PCR Clean-Up. The
entire procedure can be completed in 20 minutes, and the eluted DNA is ready to use in restriction
digestion, ligation, PCR, and sequencing reactions.

KEY FEATURES

SAMPLE SIZE: up to 300mg of agarose gel / up to 100μl of PCR products
FORMAT: Spin Column
OPERATION: Centrifuge / Vacuum Manifold
BINDING CAPACITY: 10μg DNA
EXPECTANT YIELD: 80-90% for gel extraction / 90-95% for PCR Clean-Up
OPERATION TIME: 20 minutes or less
APPLICATIONS: PCR, Fluorescent or Radioactive Sequencing, Restrictive Digestion,
DNA Labeling, Ligation

KIT CONTENTS

Component IB47010 IB47020 IB47030
DF COLUMNS 4 100 300
2ML COLLECTION TUBES 4 100 300
DF BUFFER 3ml 80ml 240ml
WASH BUFFER 1ml 25ml 50ml
(add Ethanol) (4ml) (100ml) (200ml)
ELUTION BUFFER 1ml 6ml 30ml